Chemistry and Biology of Non-canonical Nucleic Acids by Naoki Sugimoto;

Author:Naoki Sugimoto; [Sugimoto, Naoki]
Language: eng
Format: epub
ISBN: 9783527817863
Publisher: John Wiley & Sons, Inc.
Published: 2021-04-12T00:00:00+00:00

8

Replication

The main points of the learning:

Study the basis of replication in human cells.

Understand the function of non-canonical structures (especially G-quadruplex and i-motif) in replication processes.

Consider the impact of a chemical approach using small ligands and oligonucleotides toward regulation of replication.

8.1 Introduction

The genomic DNA in a cell has to be accurately copied for the daughter cells by replication at each cell cycle, which is one of the key reactions for central dogma of molecular biology. In the reaction, DNA polymerase (DNAP) with other supportive proteins initiates the reaction and synthesizes the nascent DNA strand complementary to the template DNA. Through these processes, the genomic DNA strand forming a duplex needs to be unwound for initiation of the replication reaction and should form a single-stranded state for the processivity of DNAP. Therefore, the formation of non-canonical structures along the template DNA could influence the replication. The error of replication leads to mutation and recombination of the genomic DNA, which can cause critical diseases. In this chapter, the effect of the non-canonical DNA structures, especially G-quadruplex (G4) and i-motif on the processes of replication is introduced. Although the replication machinery and pathway of replication is differed in prokaryote and eukaryote, the eukaryote especially in the case of human cell was focused in this chapter.

Download

Copyright Disclaimer:
This site does not store any files on its server. We only index and link to content provided by other sites. Please contact the content providers to delete copyright contents if any and email us, we'll remove relevant links or contents immediately.